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. 2018 Feb 16;8:3176. doi: 10.1038/s41598-018-21621-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescent staining for CD68 and iNOS. Lesion epicenter immunofluorescent staining: (A) On 1 day after SCI, the expressions of CD68 and iNOS were not significantly different. (B) On 3 day after SCI, the expressions of CD68 and iNOS were significantly suppressed by TUDCA. (C) On 7 days after SCI, the expressions of CD68 and iNOS were significantly suppressed by TUDCA (Scale bar = 80 µm). (D) Quantitative analysis of the fluorescence intensity for iNOS. (E) Analysis of the quantitative fluorescence intensity for CD68. Results are the mean ± SD of triplicate experiments: *p < 0.05, **p < 0.01, and ***p < 0.001 significant difference as compared to the control group for each time by one-way ANOVA followed by Tukey’s post hoc analysis.