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. 2018 Feb 16;8:3217. doi: 10.1038/s41598-018-21344-7

Figure 2.

Figure 2

Loss of ITK expression blocks HIV-1 replication in Jurkat cells. (A) Replication of HIV-1 in ITK expressing wild-type and non-target (n.t.) shRNA cells was compared with replication in ITK knockdown cells (258/615 shRNA). Cells were infected with an MOI 0.01 and supernatant was collected for 12 days. Virus titer was determined by infecting TZM-bl cells and measuring luciferase activity three days post infection. (B) Jurkat cells expressing ITK or no ITK were infected with single-round HIV-1 luciferase-reporter viruses pseudotyped with HIV-1 derived envelope protein (HIV-1-Env) or VSV-G protein. Luciferase activities of infected cells were determined three days post infection. (C) Jurkat wild-type cells were incubated with an ITK inhibitor (BIX02524) using different concentrations (0/2.5/5/7.5/10 µM) and transduced with HIV-1 luciferase reporter viruses either pseudotyped with HIV-1 envelope or VSV-G. Luciferase activities of infected cells were determined three days post infection. Repetition of experiments: for Fig. 2A four times; for Fig. 1B three times; for Fig. 2C two times.