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. 2018 Feb 16;8:3217. doi: 10.1038/s41598-018-21344-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of viral particles but not of gp120 envelope protein is impaired in ITK knockdown cells. Jurkat cells were incubated either with biotinylated gp120 protein (A) or GFP labeled viral particles with HIV-1 envelope (B), VSV-G envelope (C) or viral particles that were generated without any viral envelope protein (D). Data were obtained by FACS analysis of particle or protein incubated cells. Filled histograms represent negative controls composed of cells incubated without gp120 or virus. All figures show one representative experiment out of three repetitions.