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. 2018 Feb 16;8:3149. doi: 10.1038/s41598-018-21302-3

Figure 1.

Figure 1

Schematic representation of the V. cholerae strains and conditions compared in the transcriptomics analysis. V. cholerae WT and its derivative mutant strains were grown in minimal T medium with or without riboflavin as indicated. Four transcriptomics comparisons were performed. In order to identify genes whose expression is regulated by riboflavin, transcriptomes of WT in T versus WT in T plus riboflavin were compared (comparison (a)). Comparison of WT versus ΔribD (b) allowed to identify genes affected by the lack of riboflavin biosynthesis. Comparison (c) identified genes affected by the lack of riboflavin transport through RibN. Finally, comparison of the ΔribN strain with and without riboflavin pinpointed genes affected by riboflavin independently of its uptake through RibN.