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. 2018 Feb 16;8:3155. doi: 10.1038/s41598-018-21194-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Downregulation of Tnmd by knockdown of Scx in tenocytes. (a) Rat tenocytes were seeded at a density of 3 × 10⁴ cells/well in a 24-well plate and cultured to confluence. (b) At 24 h after inoculation, the cells were transfected with non-targeting siRNA (Control) or Scx siRNAs (siScx-1 or siScx-2) by lipofection. Total RNA was extracted from the cells at 72 h after lipofection and the expression levels of Scx, Tnmd, or Col1a2 were examined by qRT-PCR. The data represent the average of 3 independent experiments. The relative expression of each gene is normalized to the control and reported as the mean ± SD. ***P < 0.001 vs. control.