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. 2018 Feb 16;8:3159. doi: 10.1038/s41598-018-21484-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Heat map chart showing probability of basecalls in a particular Q-score decile being A, C, T or G. After dividing base Q-scores into deciles (as in Fig. 3), the probability that the base is A, C, T or G, given a Q-score decile was calculated. 1D and 2D data are shown for E.coli (top), while only 2D run data are shown for C. difficile (middle) and B. thailandensis (bottom). Note – rounding error may cause some groupings to appear not to sum to 1.00 even though the unrounded numbers do. (B) Averages of Q-scores per base for C. difficile, E. coli and B. thailandensis data. (C) Average data in (A) for 1D and 2D (template only) reads, broken down by organism. (D) Data in (A) for 2D consensus reads, broken down by organism.