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. 2018 Feb 16;8:3212. doi: 10.1038/s41598-018-20811-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AD-114-PA600-6H reduces migration and collagen production in fibroblast cell lines. Lung fibroblasts from IPF patients (A) IPF09 (slow-IPF) and (B) IPF14 (rapid-IPF) were plated onto BME coated wells, scratched and then layered with 2 mg/mL BME containing AD-114-PA600-6H or negative i-body. Invasion was measured for 40 h following treatment. N = 3, error bars show S.E. Statistical significance if any is shown as *p ≤ 0.05 **p ≤ 0.01 ***p ≤ 0.001 ****p ≤ 0.0001 as determined by comparing the Vehicle mean against treated mean using a Two-way ANOVA multi-comparison test, Tukey’s posthoc test. Top and bottom significance values indicate significance for cells treated with 1.66 and 4.2 µM AD-114-PA600-6H, respectively. The error bars were larger in the latter group possibly due to active senescence pathways in these cells. AD114-PA600-6H also reduced collagen protein in IPF lung fibroblasts from IPF-09 but not IPF-014 (C,D) in conditioned supernatants of fibroblast 72 h post treatment with the i-body. N = 3, error bars show S.E. Statistical significance if any is shown as *p = < 0.05 or ****p = < 0.0001 as determined following testing of Vehicle mean against treatment means using a One-way ANOVA, Dunnett’s posthoc test.