Figure 7.

Bleomycin-induced lung injury in mice is ameliorated by treatment with AD-114-Im7-FH. Mice were injected daily for 21 days with vehicle, AD-114-Im7-FH, negative i-body or AMD3100 at 10 mg/kg. (A) Body weights of each group were evaluated and are depicted. (B–E) Masson’s trichrome staining of lung histological samples from untreated healthy mice (B), mice 21 days after treatment with bleomycin (C) mice treated with bleomycin and then AD-114-Im7-FH (D) or AMD3100 (E) for 21 days. Scale bars represent 50 μm. (F) The degree of lung fibrosis was scored according to the scale defined by Ashcroft et al.³⁷. N = 5 mice per group, error bars show S.E. In A, statistical significance is shown as *p = < 0.05, as determined following testing of the Vehicle against AD-114-Im7-FH using a Two-way ANOVA multicomparison test, Tukey’s posthoc test. In F, statistical significance if any is shown as **p = < 0.01 as determined following testing of Vehicle mean against treatment means using a One-way ANOVA, Dunnett’s posthoc test.