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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: Mol Cell. 2018 Feb 15;69(4):622–635.e6. doi: 10.1016/j.molcel.2018.01.011

Figure 2. Loss of TIA1 function causes the activation of EIF2AK2-mediated stress response.

Figure 2

A) RNA-FISH and IF on DKO/FH-TIAL1 cells cultured with (+Dox) or without Dox (−Dox 6 days) to visualize poly(A)-mRNAs, FH-tagged TIAL1 and G3BP1 (Meyer et al., 2016). Nuclei stained with DAPI. Scale bar, 20 μm. B) SG-positive DKO/FH-TIAL1 cells based on representative micrographs as shown in (A). C) Polysome profiles of DKO/FH-TIAL1 cells cultured without Dox for up to 8 days. D) IB for phosphorylated EIF2S1 or EIF2AK2/PKR on DKO/FH-TIAL1 cells cultured without Dox for up to 8 days. E) IB for phosphorylated EIF2S1 on TKO/FH-TIAL1 cells cultured without Dox for up to 8 days. Lysate of DKO/FH-TIAL1 cells cultured without Dox for 8 days served as control (loaded in the left lane). F) RNA-FISH and IF on TKO/FH-TIAL1 cells cultured with (+Dox) or without Dox (−Dox 6 days) to detect poly(A)-mRNAs and FH-tagged TIAL1. Scale bar, 20 μm. G) Cell cycle analyses of TKO/FH-TIAL1 or TKO/FH-TIA1 cells cultured without Dox for up to 8 days. H) Polysome profiles of TKO/FH-TIAL1 cells cultured without Dox for up to 8 days. I) Proliferation of DKO/FH-TIAL1 or TKO/FH-TIAL1 HEK293 cells cultured with or without Dox for up to 12 days.