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. 2018 Feb 13;9:82. doi: 10.3389/fphar.2018.00082

FIGURE 4.

FIGURE 4

MiR-34a inhibitor reverses the inhibitory effects of MIAT downregulation on PC9 cells. (A) CCK-8 assay was used to determine the cell viability after miR-34a inhibitor and MIAT siRNA treatment in PC9 cells. (B) Colony formation assay was used to determine the cell growth after miR-34a inhibitor and MIAT siRNA treatment in PC9 cells. (C) Flow cytometry was used to measured cell apoptosis after miR-34a inhibitor and MIAT siRNA treatment in PC9 cells. (D) Western blot analysis for p-EGFR, EGFR, p-PI3K, PI3K, p-AKT, AKT and c-Met after miR-34a inhibitor and MIAT siRNA treatment in PC9 cells. ∗P < 0.05, ∗∗P < 0.01.