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. 2018 Feb 13;9:82. doi: 10.3389/fphar.2018.00082

FIGURE 5.

FIGURE 5

Knockdown of MIAT inhibits lung cancer growth in vivo. (A) the PC9/R cells transfected with MIAT siRNA, or together with miR-34a inhibitor were injected into nude mice. The cells transfected with empty plasmid were used as negative control. Mice were euthanized and tumors obtained from mice on day 28 after injection. (B) IHC was used to detect the expression of Ki67 in tumor section (upper), and quantification (lower). Scale bar, 100 μm. (C) TUNEL was performed to detect the apoptosis cells in tumor section (left), and the quantification of luciferase intensity (right). (D) qRT-PCR analysis for MIAT and miR-34a expression in xenografted tumor tissues. (E) Western blot analysis for c-Met and PI3K signaling proteins in xenografted tumor tissues. GAPDH was used a loading control. (F) qRT-PCR analysis for MIAT and miR-34a expression in human lung cancer tissues with high MIAT or low MIAT. ∗P < 0.05.