FIGURE 4.

Scutellarin increased cisplatin-induced autophagy. (A) A549/DDP cells were treated with the indicated concentrations of cisplatin or scutellarin, or the combination for 48 h, the expression of p62 and conversion of LC3-I to LC3-II were measured by western blotting. (B) Western blot analysis of p62 and LC3 in A549/DDP cells incubated with autophagy inhibitor HCQ. (C) Quantification of autophagic vacuoles in A549/DDP cells was analyzed by TEM. (D) The effect of HCQ on sensitivity of cisplatin and scutellarin was detected by MTT assay. Data are representative of three independent experiments (mean ± SEM). ^∗p < 0.05, ^∗∗p < 0.01.