Figure 5.

Female MRictor^−/− → Ldlr^−/− mice had smaller atherosclerotic lesions, less macrophage area and more apoptotic cells in the lesions than control Rictor^fl/fl → Ldlr^−/− mice. (A) Representative images of and Sudan IV-stained en face preparation of aortas and (B) and serial cross sections of aortic sinus stained with Oil-Red-O/hematoxylin, CD68 and TUNEL AP from Rictor^fl/fl → Ldlr^−/− mice (B, top panel) and MRictor^−/− → Ldlr^−/− mice (B, bottom panel) mice. Scale bars, 200 μm. (C,D) Quantitation of atherosclerotic lesions in aortas en face and cross sections of aortic sinus of Rictor^fl/fl → Ldlr^−/− and MRictor^−/− → Ldlr^−/− bone marrow cells; *p < 0.05 by Mann–Whitney rank sum test. (E,F) Macrophage area stained with CD68 and number of TUNEL + cells in atherosclerotic lesions of mice reconstituted with WT or MRictor^−/− bone marrow cells; *p < 0.05 by t-test.