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. 2018 Feb 13;9:144. doi: 10.3389/fpls.2018.00144

FIGURE 2.

FIGURE 2

Generation of transgenic plants and their confirmation by PCR. Different stages of transgenic plant development following tissue culture (A). PCR confirmation of putative transgenic plants using EPSPS gene specific internal forward and reverse primers. The upper amplified 1.6 kb band shows the presence of native EPSPS (with intron) gene while lower 615 bp PCR band confirms the presence of OsmEPSPS transgene (without intron) in both ‘S’ and ‘D’ transgenic groups (B).