Table 1.
Determined average concentrations of L-arginine and ADMA in human plasma ±SD (n = 3) and average recovery ±SD (n = 3) at three concentration levels obtained with sample preparation procedure (A). Recovery values in % given as mean ± SD (n = 3) obtained for procedure (B) and procedure (C) are shown for comparison.
| Human plasma, mean ± SD (µM) | Procedure A | Procedure B | Procedure C | |||
|---|---|---|---|---|---|---|
| Added (µM) | Measured, mean ± SD (µM) | Recovered, mean ± SD (%) | Recovered, mean ± SD (%) | Recovered, mean ± SD (%) | ||
| L-arginine | 65.3 ± 2.09 | 20 | 94.3 ± 10.1 | 113 ± 8.21 | — | — |
| 40 | 115 ± 3.20 | 109 ± 2.78 | 68.4 ± 5.50 | 118 ± 3.62 | ||
| 60 | 138 ± 8.67 | 110 ± 6.92 | — | — | ||
| ADMA | 0.40 ± 0.013 | 0.1 | 0.47 ± 0.030 | 91.5 ± 3.03 | — | — |
| 0.2 | 0.59 ± 0.070 | 98.0 ± 7.07 | 73.5 ± 4.22 | 94.7 ± 8.46 | ||
| 0.3 | 0.70 ± 0.032 | 98.9 ± 4.55 | — | — | ||