Table 2.
Validation data: calibration curve equation, correlation coefficient, LLOQ, ULOQ, intra- and interday precision, and average concentration ± SD quality control samples (human plasma from 6 volunteers) for L-arginine and ADMA using the selected sample preparation (A) with the developed chromatographic method.
| Calibration curve equation | Correlation coefficient (R2) | LLOQ (µM) | ULOQ (µM) | Intraday precision (CV, %) (n = 6) | Interday precision (CV, %) (n = 9) | Quality control (µM) (n = 6) | |
|---|---|---|---|---|---|---|---|
| L-arginine | y = 78686x − 3519 | 0.999 | 0.14 | 15 | 3.2 | 5.0 | 64.1 ± 10.3 |
| ADMA | y = 73655x + 1134 | 0.994 | 0.012 | 0.50 | 3.4 | 3.3 | 0.27 ± 0.02 |
| SDMA | y = 87003x + 3261 | 0.989 | — | — | 4.2 | 3.8 | 0.57 ± 0.09 |