Fig. 3.

Trajectory of HBM precursors in mouse embryos. Transverse sections of mandibular, hyoid and branchial arch levels in Wnt1-Cre/R26R-H2B-EGFP embryos at stage E10.5. Bottom panels show the magnification of pharyngeal floor. Arrowheads indicate HBM precursors. PAX3 protein was detected on both sides of the EGFP-negative basibranchial mesenchyme and laterally surrounded by EGFP-positive neural crest cells at the hyoid arch. Bbm, basibranchial mesenchyme; cncc, cardiac neural crest cells; eb1–4, ectomesenchyme of first to fourth branchial arch; eh, ectomesenchyme of hyoid arch; em, ectomesenchyme of mandibular arch; ha, hyoid arch mesoderm; oft, outflow tract; ot, otic vesicle; ra, right atrium; thy thyroid gland. Scale bars, 50 μm