FIGURE 2.

Translation from the HCV IRES in Huh-7 cells is resistant to the action of eIF4A inhibitors. Huh-7 cells were transfected with reporter RNAs (HCV-Luc, EMCV-Luc, or CrPV-Luc) for 1 h and then treated with either Hipp (0.2 and 0.4 μM) or Pat A (0.2 and 0.4 μM) for 2 h, after which luciferase activity was measured. Bars represent the relative luciferase activity with non-treated control (inhibitor concentration = 0) set as 100%. The readings from CHX treatments were subtracted from all as a baseline. Error bars represent the standard error of the mean, n = 3. Statistical significance of the differences between treated samples compared to control was calculated with two-way ANOVA and a Bonferroni post hoc test, and is shown as: ^∗∗p < 0.01, ^∗∗∗p < 0.001.