Table 1.

Dissociation Constants for P450cam Complexes

variant	substrate	KD (μM)	ΔG (kcal/mol)
WT	camphor	1.8 ± 0.8	−7.8 ± 0.3
WT	isoborneol	10.9 ± 3.4	−6.8 ± 0.2
WT	camphane	18.7 ± 2.5	−6.4 ± 0.1
Y96CNF	camphor	3.5 ± 1.0	−7.4 ± 0.2
Y96CNF	isoborneol	5.7 ± 1.7	−7.1 ± 0.2
Y96CNF	camphane	6.4 ± 1.8	−7.1 ± 0.2
Y96F	camphor	3.5 ± 0.6	−7.4 ± 0.1
Y96F	isoborneol	1.2 ± 0.3	−8.1 ± 0.2
Y96F	camphane	10.7 ± 3.3	−6.8 ± 0.2