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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Anal Biochem. 2017 Nov 24;542:63–75. doi: 10.1016/j.ab.2017.11.018

Figure 7. Aggregation inhibition determination using detergent screening.

Figure 7

Residual activity of YpIspC (white) or FtIspD (dark grey) in the absence (open bars) and presence (hatched bars) of 0.01% Triton X-100. All samples are standardized to an uninhibited control containing the vehicle (DMSO) and 0.01% Triton X-100 if necessary. FR900098 was used as a positive control for specific inhibition. The cut-off for significant attenuation of inhibition was set as 25% or greater increase in residual enzyme activity; compounds with significantly attenuated activity in the presence of Triton X-100 are indicated with a star. Error bars are calculated as the deviation from the mean of two independent measurements. A) Inhibition is attenuated in the presence of Triton X-100 for all inhibitors except sanguinarine chloride, and inhibitor of IspC. B) Inhibition is attenuated in the presence of Triton X-100 for all inhibitors except suramin hexasodium, an inhibitor of IspC, and 6-hydroxy DL DOPA, an inhibitor of IspD.