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. 2018 Feb 14;9:220. doi: 10.3389/fimmu.2018.00220

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Copyright © 2018 Splichalova, Slavikova, Splichalova and Splichal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoblotting analysis of claudin-1 in the colon of the preterm and the term germ-free piglets. The expression of claudin-1 (normalized to β-actin) in the colon of the preterm (n = 10) and the term (n = 6) germ-free piglets was analyzed by Western blot. Examples of β-actin (upper) and claudin-1 (lower) bands detected in one sample from each group are shown in the columns. The results are depicted as mean + SEM. The statistical significances are denoted with double asterisks (P < 0.01).