Figure 1.

Inhibition of ABCC-like activity by MK-571 in epimastigote forms of the T. cruzi Y strain. ABCC-like activity was evaluated by the carboxyfluorescein (CF) efflux assay. 10⁶ epimastigotes were incubated in medium containing 5 μM CFDA in the presence or absence of 100, 200, or 300 μM MK-571 for 30 min. Parasites were then centrifuged and incubated in medium in the presence or absence of MK-571 for another 30 min. After incubation, parasites were centrifuged, suspended in PBS and kept on ice for acquisition by flow cytometry. Graphs in (A,B) represent the median fluorescence intensity (MFI) for CF, (C,D) the percentages of CF+ parasites at 27 and 37°C, respectively. The Friedman statistical test was used with n = 10 (27°C) and n = 8 (37°C) independent experiments. Lines represent the median for each group and the significance values were represented by ^*p < 0.05; ^**p < 0.01, and ^***p < 0.001.