Figure 4.

Inhibition of ABCC-like activity after ATP depletion in epimastigote forms of the T. cruzi Y strain. ABCC-like activity was evaluated by the carboxyfluorescein (CF) efflux assay. 10⁶ epimastigotes were preincubated for 60 min at 27 or 37°C with 40 mM sodium azide (NaN₃) or 1 mM iodoacetic acid (C₂H₃IO₂) in PBS. Parasites were then centrifuged and suspended in PBS containing 5 μM CFDA. After 30 min, parasites were centrifuged and incubated in PBS for another 30 min. After incubation, parasites were centrifuged, suspended in PBS and kept on ice for acquisition by flow cytometry. Graphs shown in (A,B) represent the CF MFI, (C,D) the percentages of CF+ parasites at 27 and 37°C, respectively. The Friedman statistical test was used with n = 8 independent experiments. Lines represent the median for each group and the significance values were represented by ^*p < 0.05; ^**p < 0.01, and ^***p < 0.001.