Figure 5.

Reduced ABCC-like activity in absence of an energy source in epimastigote forms of the T. cruzi Y strain. ABCC-like activity was evaluated by the carboxyfluorescein (CF) efflux assay. 10⁶ epimastigotes were incubated in medium in the presence or absence of glucose containing 5 μM CFDA in the accumulation step. After 30 min, parasites were centrifuged and incubated in RPMI or PBS for another 30 min. After incubation, parasites were centrifuged, suspended in PBS and kept on ice for acquisition by flow cytometry. The Friedman statistical test was used with n = 8 independent experiments. Bars represent the mean ± standard error and the significance values were represented by ^**p < 0.01 and ^***p < 0.001.