Figure 6.

Depletion of non-protein thiols in epimastigote forms of the T. cruzi Y strain. 10⁶ epimastigotes were preincubated with 100 μM N-ethylmaleimide (NEM) in PBS for 60 min or with 3 mM buthionine sulfoximine (BSO) in medium for 48 h at 27°C. Then, parasites were centrifuged and suspended in PBS containing 1 μM CMFDA at 27 or 37°C. After 15 min of incubation, parasites were centrifuged, suspended in PBS and kept on ice for acquisition by flow cytometry. Graphs shown in (A,B) represent the median fluorescence intensity (MFI) for TMF after preincubation with NEM, (C,D) the TMF MFI after preincubation with BSO at 27 and 37°C, respectively. The Wilcoxon statistical test was used with 11 ≤ n ≤ 13 independent experiments. Lines represent the median for each group and the significance values were represented by ^***p < 0.001.