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. 2018 Jan 1;8(4):1084–1105. doi: 10.7150/thno.21740

Figure 4.

Figure 4

Effects of OIP5-AS1 and miR-367-3p on the biological behavior of glioma cells: over-expression of OIP5-AS1 elevated CEBPA expression via down-regulating miR-367-3p. (A) CEBPA protein expression levels in normal brain tissues (NBT), low-grade, and high-grade glioma using GAPDH as an endogenous control. Representative protein expression and their integrated density values (IDVs) of CEBPA in NBT, low-grade glioma tissues (WHO I-II), and high-grade glioma (WHO III-IV) are shown (data are presented as the mean ± SD (n = 15, each group);**P < 0.01 relative to NBTs group; ##P < 0.01 relative to low-grade glioma tissues group). (B) CEBPA protein expression levels in normal human astrocytes (NHA), U87 and U251 cells using GAPDH as an endogenous control. Representative protein expressions and their IDVs in NHA, U87 and U251 are shown (data are presented as the mean ± SD (n = 3, each group);**P < 0.01 relative to NHA group). (C, D) Western blot analysis forOIP5-AS1 and miR-367-3p-regulatedCEBPA expression using GAPDH as endogenous control (data are presented as the mean ± SD (n = 3, each group);**P < 0.01 relative to sh-NC+pre-NC group). (E) Cell Counting Kit-8 (CCK-8) assay was used to evaluate the effect of OIP5-AS1 and miR-367-3p on U87 and U251 cells' proliferation. (F) Flow cytometry analysis of U87 and U251 cells with altered expression of OIP5-AS1 and miR-367-3p (data are presented as the mean ± SD (n =3, each group);**P < 0.01 relative to sh-NC+pre-NC group). (G) Quantification of number of migrating and invading cells with altered expression of OIP5-AS1 and miR-367-3p. Representative images and corresponding statistical plots are presented (scale bars represent 80μm).