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Short-term and long-term effects of Shh gene transfer on IR-induced cellular senescence in SMGs. A-B: SA-βgal staining of SMG sections 7 days after IR and the quantification of SA-βgal+ area (N=6). C-D: qRT-PCR analysis on mRNA expression of senescence marker genes 7 or 90 days after IR with or without intragland delivery of GFP or Shh gene. N = 3. *: P < 0.05 vs. IR unless labeled otherwise.
