Figure 6.

Forced expression of let-7d ameliorated cardiac fibrosis in the mice after MI. (A) Tail vein injection of Adv-let-7d increased let-7d expression in normal mice, as measured by qRT-PCR; GAPDH served as an internal control, and Adv-NC served as a negative control. (B) Representative images of Masson-stained hearts of MI mice after injection of Adv-let-7d; quantification of the total fibrotic area using Image-Pro Plus. Forced expression of let-7d restored the impaired cardiac function in infarcted hearts (C-D), ameliorated collagen production (E) and reversed the induction of fibrosis-related protein expression (F). n=13 mice per group for the heart function evaluation; and n=6 mice for the qRT-PCR and western blot assays. *p<0.05 and **p<0.01 vs. control or sham mice; ^#p<0.05 and ^##p<0.01 vs. MI mice.