FIGURE 5.

Location of SGLT2 in a brain tumor using immunocytochemistry

IHC was carried out to examine the distribution of SGLT2 in a grade IV astrocytoma. A: the specific SGLT2 immunoreaction found in a tumor microvessel, the surrounding glia, and macrophages and cancer cells. The antigen used to generate the SGLT2 polyclonal antibody blocked the reactivity (not shown). B: an adjacent section stained with the glial marker CD163. The results suggest that Me-4FDG gains access to the brain through SGLT2 expressed in the microvasculature of the tumor where it is accumulated by SGLT2 in the plasma membrane of tumor cells. See Ref. 24.