Table 2. Kinetic characterization of MVKs.
MVK | Km-Mev (μM) | Km-ATP (μM) | MW (Da) | Specific activity µmol/min*mg | kcat (s−1) | kcat /Km-Mev (µM/s) |
---|---|---|---|---|---|---|
mcl | 17.0±0.5 | 74±1 | 35 626 | 233±2 | 14.0±0.1 | 0.824 |
mpd | 15.0±0.5 | 119±2 | 33 908 | 129±1 | 7.30±0.04 | 0.487 |
mma | 83±2 | 687±9 | 33 575 | 348±3 | 19.0±0.2 | 0.229 |
nmr | 461±7.5 | 1006±20 | 36 594 | 204±2 | 12±0.11 | 0.026 |
sce | 73±2 | 464±8 | 50 622 | 188±3 | 16.0±0.1 | 0.219 |