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. 2017 Sep 4;163(9):1306–1318. doi: 10.1099/mic.0.000516

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© 2017 The Authors

This is an open access article under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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Fig. 2. — SPase-related mutants show different patterns of biofilm formation. Bacterial samples of eight replicates each were cultured anaerobically for 24 h in BM with 1 % sucrose. After crystal violet staining, biofilm formation was quantified at OD₆₀₀ and the results were compared using ANOVA and the multiple comparison method (Dunnett's test). ** indicates significance with P-value <0.01.