Table 2. Differentially expressed proteins in ΔSSA_0351 using mass spectrometry.
| Protein symbol | Fold change | Score* | Protein description | SPase substrate† |
|---|---|---|---|---|
| SSA_1748 | 0.00000234 | 5.61 | Probable manganese-dependent inorganic pyrophosphatase | No |
| SSA_1938 | 0.05 | 5.43 | Enoyl-[acyl carrier protein] reductase II | No |
| SSA_1498 | 0.12 | 12.34 | 50S ribosomal protein L20 | No |
| SSA_2107 | 0.17 | 10.71 | Glutamine–fructose-6-phosphate aminotransferase | No |
| SSA_0570 | 0.23 | 5.46 | Glutamyl-tRNA(Gln) amidotransferase subunit A | No |
| SSA_0291 | 0.31 | 5.53 | Short-chain dehydrogenase | Yes |
| SSA_1012 | 0.42 | 5.99 | Phosphoenolpyruvate–protein phosphotransferase | No |
| SSA_2342 | 0.57 | 5.12 | VTC domain protein | No |
| SSA_2359 | 0.63 | 10.02 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG | No |
| SSA_1528 | 0.63 | 5.16 | Phosphoglycerate mutase | No |
| SSA_0371 | 0.64 | 10.9 | Glutamate dehydrogenase | No |
| SSA_2183 | 0.76 | 28.3 | Glucose-6-phosphate isomerase | No |
| SSA_0126 | 0.8 | 26.5 | 50S ribosomal protein L15 | No |
| SSA_2126 | 0.97 | 5.44 | TatD family deoxyribonuclease | No |
| SSA_2069 | 1.06 | 5.79 | Proline–tRNA ligase | No |
| SSA_0117 | 1.14 | 58.35 | 50S ribosomal protein L14 | No |
| SSA_1998 | 1.14 | 32.74 | Trigger factor | No |
| SSA_0357 | 1.2 | 12.02 | Thioredoxin | No |
| SSA_0226 | 1.34 | 56.08 | 60 kDa chaperonin | No |
| SSA_2075 | 1.53 | 5.49 | Transketolase | No |
| SSA_0848 | 1.59 | 149.33 | Pyruvate kinase | No |
| SSA_2007 | 2.06 | 39.14 | Chaperone protein DnaK | No |
| SSA_1968 | 2.21 | 11.34 | Ketol-acid reductoisomerase | No |
| SSA_1836 | 2.25 | 11.05 | Competence protein FA | No |
| SSA_0110 | 2.26 | 130.27 | 50S ribosomal protein L2 | No |
| SSA_0847 | 2.34 | 27.94 | ATP-dependent 6-phosphofructokinase | No |
| SSA_1948 | 2.35 | 11.69 | Oligopeptide-binding protein SarA | No |
| SSA_0768 | 2.35 | 16.49 | Ribonucleoside-diphosphate reductase | No |
| SSA_0132 | 2.39 | 27.09 | DNA-directed RNA polymerase subunit alpha | No |
| SSA_2202 | 2.43 | 40.83 | Elongation factor Ts | No |
| SSA_0440 | 2.54 | 32.15 | 30S ribosomal protein S18 | No |
| SSA_1520 | 2.56 | 195.25 | Elongation factor Tu | No |
| SSA_0683 | 2.57 | 91.7 | DNA-binding protein HU | No |
| SSA_0141 | 2.72 | 5.57 | Copper chaperone | No |
| SSA_1032 | 2.85 | 7.03 | 30S ribosomal protein S20 | No |
| SSA_0098 | 3.04 | 5.18 | HAD superfamily hydrolase | No |
| SSA_0130 | 3.2 | 30.68 | 30S ribosomal protein S13 | No |
| SSA_0112 | 3.3 | 24.53 | 50S ribosomal protein L22 | No |
| SSA_1204 | 3.4 | 23.43 | Phosphoglucomutase | No |
| SSA_1925 | 3.5 | 11.65 | Serine–tRNA ligase | No |
| SSA_1950 | 3.81 | 11.79 | Oligopeptide ABC superfamily ATP binding cassette transporter, binding protein | No |
| SSA_0774 | 4.02 | 224.38 | Glyceraldehyde-3-phosphate dehydrogenase | No |
| SSA_1992 | 4.25 | 171.16 | Fructose-bisphosphate aldolase | No |
| SSA_0886 | 4.58 | 168.8 | Enolase | No |
| SSA_0302 | 4.74 | 89.05 | Phosphoglycerate kinase | No |
| SSA_2033 | 4.75 | 37.55 | 30S ribosomal protein S9 | No |
| SSA_1619 | 4.79 | 5.67 | Ribosome-recycling factor | No |
| SSA_1839 | 4.83 | 57.73 | Cysteine synthase | No |
| SSA_1221 | 4.98 | 22.09 | l-lactate dehydrogenase | Yes |
| SSA_0176 | 5.07 | 21.1 | DNA-directed RNA polymerase subunit beta | No |
| SSA_1062 | 5.75 | 11.69 | 50S ribosomal protein L27 | No |
| SSA_0688 | 5.77 | 32.78 | 2,3-bisphosphoglycerate-dep phosphoglycerate mutase | No |
| SSA_0859 | 7.75 | 62.77 | Triosephosphate isomerase | No |
| SSA_0813 | 9.7 | 11.04 | Thioredoxin reductase | No |
| SSA_0772 | 9.91 | 75.4 | PTS family porter, phosphocarrier protein HPR | No |
| SSA_0878 | 11.11 | 16.27 | DNA gyrase subunit B | No |
| SSA_1896 | 11.8 | 32.13 | Translation initiation factor IF-2 | No |
| SSA_1043 | 12.99 | 5.47 | Homoserine dehydrogenase | No |
| SSA_0636 | 14.5 | 6.01 | N-(5′-phosphoribosyl)anthranilate isomerase | No |
| SSA_0437 | 14.64 | 23.25 | 30S ribosomal protein S6 | No |
| SSA_0522 | 48.94 | 10.91 | Putative ethanolamine utilization protein EutM1 | No |
*Score provided by Progenesis software. By default, a score >5 indicates a statistically significant finding according to the following parameters for protein identification: allowed missed cleavages by trypsin set to 1; fixed modifications by carbamidomethyl (C); variable oxidation (M) was allowed; at least 2 peptides per protein; 4 fragments per peptide; 10 fragments per protein.
†As predicted by LipoP 1.0 software.