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. 2018 Jan 4;2018:2821040. doi: 10.1155/2018/2821040

Figure 4.

Figure 4

Immunohistochemistry, western blot analysis, and gene expression analysis of redox status in the hippocampal area. Immunohistochemistry analysis against the 4-HNE (a). Western blot analysis of iNOS and 4-HNE in the hippocampal regions (b) and their protein intensities (c, d). Gene expression analysis of antioxidant-related molecules including GSS, Grd, Gpx, SOD-1, SOD-2, and SOD-3 (e). Data are expressed as the mean ± SD (n = 2 for ICH analysis, n = 8 to 10 for western blot analysis, and n = 4 to 5 for gene expression analysis). p# < 0.05 and p## < 0.01 versus normal group; p∗ < 0.05 versus scopolamine only injection group. 4-HNE: 4-hydroxyneal, GSS: glutathione-synthase, Grd: glutathione-reductase, Gpx: glutathione-peroxidase, and SOD: superoxide dismutase.