Table 2.

Sequence of the primers used in real-time PCR analysis.

Gene list (NCBI number)	Primer sequencing (forward and reverse)	Product size (base pair)	Annealing temperature (°C)
GSS (NM-001291111)	5′-ACC GAA GGC TGT TTA TGG ATG A-3′	100	59
	5′-AGG CGT GCT TCC CAG TTC T-3′
Grd (NM-010344.4)	5′- GAT GTG TGG AGC GGT AAA CTT TT -3′	120	60
	5′- AGC CGC CRG AAC ACC ATC TA -3′
Gpx (NM-001329527.1)	5′-CTC ACC ATT CAC TTC GCA CTT C-3′	122	59
	5′-ACA CCA GGA GAA TGG CAA GAA-3′
SOD-1 (NM_011434)	5′-TGT CAG GAC AAA TTA CAG GAT TAA CTG-3′	100	60
	5′-AAA TGA GGT CCT GCA CTG GTA CA-3′
SOD-2 (NM_013671)	5′-CCCAGACCTGCCTTACGACTAT-3′	112	58
	5′-GGTGGCGTTGAGATTGTTCA-3′
SOD-3 (NM_011435)	5′-GGT GGA TGC TGC CGA GAT-3′	101	59
	5′-GCT GCC GGA AGA GAA CCA A-3′
SOD-3 (NM-011435)	5′-GGT GGA TGC TGC CGA GAT-3′	101	59
	5′-GCT GCC GGA AGA GAA CCA A-3′
mAChR 1 (NM-001112697)	5′-AGT GGC ATT CAT CGG GAT CA-3′	100	60
	5′-CTT GAG CTC TGT GTT GAC CTT GA-3′
CBP (NM-001025432)	5′-CTG GCA GAC CTC GGA AAG AA-3′	100	59
	5′-CTG GCG CCG CAA AAA CT-3′
CREB 1 (NM-013497)	5′-ACA GTG CCA ACC CCC ATT TA-3′	100	59
	5′-GTA CCC CAT CCG TAC CAT TGT T-3′
BDNF (NM-001048139)	5′-CAC TTT TGA GCA CGT CAT CGA A-3′	104	60
	5′-CAC CCG GGA AGT GTA CAA GTC-3′
GAPDH NM_001289726	5′-TCA CTC AAG ATT GTC AGC AAT GC-3′	100	58
	5′-GGC CCC GGC CTT CTC-3′

BDNF: brain-derived neurotrophic factor, CREBP: cAMP response element-binding protein, CBP: CREBP binding protein, GSS: glutathione synthase, Grd: glutathione-reductase, Gpx: glutathione-peroxidase, mAchR1: muscarinic acetylcholine receptor 1, SOD: superoxide dismutase.