Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 23;30(1):e12563. doi: 10.1111/jne.12563

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors. Journal of Neuroendocrinology published by John Wiley & Sons Ltd on behalf of British Society for Neuroendocrinology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

High‐fat diet (HFD) feeding induces elevations in ventromedial hypothalamus (VMH) norepinephrine and serotonin activity. Female Sprague‐Dawley (SD) rats (4 week old, n = 7‐9 per group) were fed either a HFD or rodent chow (RC) diet for 6 weeks and then 24‐h in vivo VMH extracellular profiles of monoamine metabolites were investigated by 24‐h microdialysis sampling in each group. Daily profiles of (A) NE, (B) MHPG and (C) 5‐HIAA in microdialysate samples from VMH of freely‐moving rats fed either HFD (●) or RC (○) (n = 7 or 8 per group). The horizontal bar indicates light and dark phases. HFD fed rats exhibited elevated levels of VMH 3‐methoxy‐4‐hydroxyphenylglycol (MHPG), norepinephrine (NE) and 5‐HIAA compared to RC diet rats (by 38%, 63% and 48%, respectively). Repeated measures anova on NE indicated a group effect (F _1,13 = 14.01, P < .005), as did repeated measures anova on MHPG (F _1,13 = 5.334, P < .05). Repeated measures anova on 5‐HIAA also revealed a group effect (F _1,13 = 7.630, P < .05)