Fig. 5.

Analysis of polyketide in culture filtrate extracts from M. oryzae and phytotoxic activity of extracts and pure compounds. (a) HPLC chromatograms of extracts from MoWT, ΔMopks19, ΔMopks19/MoPKS19, ΔMoC19oxr1 and the overexpression mutant MoEF1 :: C19OXR1. The M. oryzae cultures were grown as described in Methods. Samples were taken 8 h after transfer to REM. The HPLC analysis was conducted using extracts of the culture broth (210 nm wavelength). mAU, Milli-absorption units. Phytotoxic activity of the extracts towards rice leaves is shown below each chromatogram. The assays were conducted as described in Methods. (b) Phytotoxicity was monitored for the pure compounds under equal conditions.