Table 2.
Effect of different plant growth regulators on shoot multiplication from in vitro raised shoot tips of A. benthamii on MS ×1/2 medium.
| BAP (µM) | TDZ (µM) |
IAA (µM) | NAA (µM) |
Shoot number |
Shoot length (cm) |
Callusing (%) |
Number of days for minimum shoot formation (n = 3) | Minimum shooting response (%) |
|---|---|---|---|---|---|---|---|---|
| - | - | - | - | - | - | - | - | |
| 1.5 | 1.0 | 3.1 ± 1.07a | 2.0 ± 0.97a | +++ | 55 | 80 | ||
| 2.5 | 2.0 | - | 5.3 ± 0.87b | 2.3 ± 1.32a | + | 53 | 80 | |
| 3.5 | 3.0 | - | 8.3 ± 2.9c | 3.5 ± 1.1b | + | 44 | 75 | |
| 4.5 | 4.0 | - | 9.1 ± 2.13c | 3.4 ± 0.76b | + | 40 | 70 | |
| 5.5 | 5.0 | - | 3.2 ± 0.56ab | 2.1 ± 0.63a | + | 50 | 80 | |
| 6.5 | 6.0 | - | 1.1 ± 0.16a | 2.0 ± 0.87a | +++ | - | - | |
| 3.5 | 3.0 | 4.5 ± 1.3b | 2.9 ± 0.13ab | +++ | 50 | 90 | ||
| 4.5 | 4.0 | 8.5 ± 3.2c | 3.3 ± 1.3b | ++ | 45 | 90 | ||
| 5.5 | 5.0 | 11.0 ± 3.1d | 3.6 ± 0.76b | ++ | 45 | 85 | ||
| 6.5 | 6.0 | 12.4 ± 2.6d | 4.4 ± 1.98b | ++ | 35 | 85 | ||
| 7.5 | 7.0 | 11.2 ± 2.1d | 3.65 ± 2.1a | ++ | 30 | 80 | ||
| 8.5 | 8.0 | 9.7 ± 1.4c | 3.0 ± 1.5b | ++ | 46 | 80 | ||
| 9.5 | 9.0 | 5.6 ± 1.65b | 1.3 ± 0.33a | +++ | 52 | 90 | ||
| 10.5 | 10.0 | 2.2 ± 0.8a | 1.25 ± 0.78a | +++ | - | - | ||
| 1.0 | 0.5 | 9.0 ± 1.8c | 3.13 ± 1.8b | + | 40 | 90 | ||
| 2.0 | 1.0 | 14.2 ± 2.6d | 3.32 ± 1.32b | ++ | 37 | 100 | ||
| 3.0 | 1.5 | 20.1 ± 3.9e | 5.6 ± 1.6c | + | 28 | 100 | ||
| 4.0 | 2.0 | 16.0 ± 2.0de | 5.3 ± 0.5c | + | 27 | 100 | ||
| 5.0 | 2.5 | 12.03 ± 1.4d | 4.8 ± 0.32b | + | 35 | 90 | ||
| 6.0 | 3.0 | 8.9 ± 2.3c | 3.0 ± 0.97b | + | 37 | 90 | ||
| 7.0 | 3.5 | 5.2 ± 1.2b | 3.0 ± 1.0b | + | 40 | 90 | ||
| 8.0 | 4.0 | 3.2 ± 1.6ab | 2.7 ± 1.43a | ++ | 40 | 90 | ||
| 3.0 | 1.5 | 10.5 ± 2.0cd | 3.0 ± 1.3b | ++ | 44 | 80 | ||
| 4.0 | 2.0 | 13.0 ± 1.5d | 3.5 ± 1.9b | ++ | 35 | 80 | ||
| 5.0 | 2.5 | 15.3 ± 1.09d | 4.8 ± 1.5b | + | 30 | 90 | ||
| 6.0 | 3.0 | 9.9 ± 2.76c | 4.4 ± 1.2b | ++ | 30 | 95 | ||
| 7.0 | - | 3.5 | 5.3 ± 1.23b | 3.1 ± 0.56b | ++ | 38 | 85 |
Note. Data scored after 12 weeks of culture period. Data represented as mean ± SD (n = 10); + = low intensity callus formation; ++ = moderate intensity callus formation; +++ = high intensity callus formation. Data was statically analyzed using Duncan multiple range test by SPSS 17.0 software. The values followed by different superscripts are statically significant with each other at P < 0.05. TDZ: thidiazuron; IAA: indole 3-acetic acid; Kn: kinetin; BAP: 6-benzylamino purine; IBA: indole 3-butyric acid.