Table 3.
Effect of different concentrations of auxins (IBA/IAA) with TDZ on root regeneration of Arnebia benthamii from multiple shoots.
| TDZ (µM) | IBA (µM) | IAA (µM) | Root number | Callusing (%) | Rooting response (%) |
|---|---|---|---|---|---|
| 1.0 | 0.5 | - | - | - | |
| 2.0 | 1.0 | - | - | - | |
| 3.0 | 1.5 | - | 1.0 ± 0.12a | ++ | 70 |
| 4.0 | 2.0 | - | 1.8 ± 0.55a | +++ | 80 |
| 5.0 | 2.5 | - | 2.5 ± 0.76b | ++ | 80 |
| 6.0 | 3.0 | - | 2.6 ± 0.3a | ++ | 85 |
| 7.0 | 3.5 | - | 3.7 ± 0.2bc | ++ | 85 |
| 8.0 | 4.0 | - | 5.3 ± 0.0c | + | 65 |
| 1.0 | - | 0.5 | 1.9 ± 0.6ab | ++ | 80 |
| 2.0 | - | 1.0 | 3.2 ± 0.5b | +++ | 80 |
| 3.0 | - | 1.5 | 8.3 ± 0.0d | - | 85 |
| 4.0 | - | 2.0 | 7.4 ± 0.0cd | - | 70 |
| 5.0 | - | 2.5 | 6.0 ± 0.0c | - | 80 |
| 6.0 | - | 3.0 | 3.3 ± 0.0b | + | 70 |
| 7.0 | - | 3.5 | 2.12 ± 0.5b | + | 65 |
| 8.0 | - | 4.0 | 0.5 ± 0.04a | +++ | 75 |
Data scored after 12 weeks of culture period. Data represented as mean ± SD (n = 10); + = low intensity callus formation; ++ = moderate intensity callus formation; +++ = high intensity callus formation. Data was statically analyzed using Duncan multiple range test by SPSS 17.0 software. The values followed by different superscripts are statically significant with each other at P < 0.05. TDZ: thidiazuron; IAA: indol 3-acetic acid; IBA: indole 3-butyric acid.