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For the spectral library IT DDA, OT DDA, and untargeted DIA acquisition resulted in A) 27 897, 33 673, and 17 894 unique peptide sequences within the 400–800 m/z range and B) 4770, 4989, and 2079 protein groups, respectively. From the merged spectral library, which contains all peptides identified by either IT, OT, or untargeted DIA; C) 31 466 and 24 570 unique peptide sequences; and D) 4578 and 4071 protein groups were quantified by DIA and WiSIM‐DIA, respectively.
