Table 4.
Inhibitory effects of the Sephadex® fractions on AGEs formation in vitro, induced by glucose and methylglyoxal.
| Glucose-induced AGEs (IC50a, mg/ml) |
Methylglyoxal-induced AGEs (% inhibitionb) |
||
|---|---|---|---|
| Fluorescent AGEs | CML, Pent, others | Fluorescent AGEs | |
| AG | 0.12 | 0.52 | 94.09 ± 0.10c (IC50 = 0.34 mg/ml) |
| TM-CW | 5.30 | 0.02 | 27.79 ± 1.00d |
| HMW | >10.00 | 0.002 | 13.18 ± 0.54e |
| MMW | 7.70 | 0.001 | 16.43 ± 1.19f |
| LMW | 5.20 | 4.00 | 38.03 ± 0.68g |
Fluorescent AGEs were quantified at 445 nm (λexc 370 nm). CML, Pent, and other AGE structures were measured using ELISA kit. aHalf maximal inhibitory concentration (IC50) was determined from concentration-response curve of two independent experiments. bPercentage AGEs inhibition of sample was calculated at 5 mg/ml (mean ± SD, n = 2). Means in the same column with different letters (c–g) are significantly different (p < 0.05). CML, N𝜀-(carboxymethyl)lysine; Pent, pentosidine; AG, aminoguanidine hydrochloride; TM-CW, L. rhinocerus TM02 sclerotial cold water extract; HMW, high-molecular-weight fraction; MMW, medium-molecular-weight fraction; LMW, low-molecular-weight fraction.