Table 5.
Polyphenols in orange fruit pericarp of transgenic lines and WT.
Chlorogenic acid (μg/gdwt) | SE | p | Caffeic acid glucoside (arbitrary units) | SE | p | Cis-chlorogenic acid (arbitrary units) | SE | p | Quercetin derivative (arbitrary units) | SE | p | Rutin (μg/gdwt) | SE | p | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | 3245.2 | 31.2 | ab | 3.5 | 0.0 | a | 1.3 | 0.0 | ab | 0.4 | 0.00 | a | 435.5 | 2.9 | a |
AO | 2537.5 | 81.5 | a | 3.0 | 0.2 | a | 1.0 | 0.1 | a | 0.4 | 0.02 | a | 397.1 | 8.1 | a |
GLD | 3029.0 | 298.5 | ab | 3.2 | 0.5 | a | 1.1 | 0.1 | ab | 0.4 | 0.01 | a | 406.0 | 21.4 | a |
MDHAR | 3554.4 | 68.3 | b | 3.7 | 0.2 | a | 1.5 | 0.1 | b | 0.3 | 0.01 | a | 446.7 | 21.3 | a |
Polyphenolic compounds were assayed in the pericarp tissue (3 biological replicates of 30 fruits per pool) of the lines by HPLC-DAD. When the purified compound was not available for exact quantification, arbitrary units corresponding to peak surface were used. Measurements show means with standard error (SE). A comparison of means was carried out using a Kruskal Wallis test with correction (Dunn). Different letters indicate significant differences (5% significance level).