Table 6.
Proteins in orange fruit pericarp of the lines as identified by mass spectrometry.
Spot ID | ITAG2.4 | Annotated function | Significant difference in lines | Fold change of transcript AO vs. MDHAR | p-value (FDR) | Correlation transcript-protein |
---|---|---|---|---|---|---|
CM148 | Solyc01g099190.2.1 | Lipoxygenase | No data | No data | ||
MP25 | Solyc01g103450.2.1 | Chaperone DnaK | MDHAR down, AO up | |||
MP26 | Solyc01g104920.2.1 | 26S protease regulatory subunit 8 homolog | −1.84 | 0.002 | ||
MP14 | Solyc01g111300.2.1 | Cold shock protein-1 | ||||
MP31 | Solyc02g070510.2.1 | Proteasome subunit alpha type | No data | No data | ||
MB16 | Solyc02g080630.2.1 | Lactoylglutathione lyase | MDHAR down | |||
MP18 | Solyc02g085790.2.1 | T-complex protein 1 subunit zeta | 5.82 | 0.005 | ||
MP38 | Solyc02g091100.2.1 | Oxalyl-CoA decarboxylase | ||||
MP42 | Solyc02g092670.1.1 | Subtilisin-like protease | No data | No data | ||
JC65 | Solyc03g097270.2.1 | Cysteine proteinase inhibitor | MDHAR up | −1.40 | 0.017 | Yes |
MP24 | Solyc04g045340.2.1 | Phosphoglucomutase | ||||
MP29 | Solyc04g045340.2.1 | Phosphoglucomutase | ||||
CM57 | Solyc05g005490.2.1 | Carbonic anhydrase | MDHAR up, AO down | 1.98 | 0.008 | No |
MP17 | Solyc05g012580.1.1 | Unknown Protein | ||||
MP11 | Solyc05g054580.2.1/Solyc09g091520.1.1 | 60S acidic ribosomal protein P0 | MDHAR up | −1.34 | 0.023 | Yes |
JC26 | Solyc06g005160.2.1 | Ascorbate peroxidase | 1.25 | 0.036 | ||
MP13 | Solyc06g005940.2.1 | Protein disulfide isomerase | ||||
MP33 | Solyc06g005940.2.1 | Protein disulfide isomerase | ||||
JC60 | Solyc06g059740.2.1 | Alcohol dehydrogenase 2 | 1.49 | 0.004 | ||
TP17 | Solyc06g059740.2.1 | Alcohol dehydrogenase 2 | 1.49 | 0.004 | ||
MP20 | Solyc06g060290.2.1 | Protein disulfide isomerase | MDHAR up, AO down | No data | No data | |
MP36 | Solyc06g065270.2.1 | Adenylate kinase | ||||
TP55 | Solyc06g076520.1.1 | Class I heat shock protein | MDHAR up | |||
CM94 | Solyc07g005820.2.1 | Heat shock protein 70 | MDHAR down | |||
MP23 | Solyc07g066600.2.1 | Phosphoglycerate kinase | −1.36 | 0.033 | ||
MP22 | Solyc08g014130.2.1 | 2-isopropylmalate synthase 1 | 2.05 | 0.000 | ||
JC79 | Solyc08g014130.2.1 | 2-isopropylmalate synthase 1 | 2.05 | 0.000 | ||
MP21 | Solyc08g062660.2.1 | Ran GTPase binding protein | No data | No data | ||
MP41 | Solyc09g082060.2.1 | Cysteine synthase | ||||
MP37 | Solyc09g083410.2.1 | Amidase hydantoinase/carbamoylase family protein | MDHAR down | No data | No data | |
MP19 | Solyc09g090330.2.1 | Harpin binding protein 1 | No data | No data | ||
MP12 | Solyc09g098150.2.1 | Metacaspase 7 | MDHAR up, AO down | 2.01 | 0.003 | No |
MP16 | Solyc09g098150.2.1 | Metacaspase 7 | 2.01 | 0.003 | ||
MP32 | Solyc09g098150.2.1 | Metacaspase 7 | 2.01 | 0.003 | ||
MP34 | Solyc10g081240.1.1 | Protein grpE | ||||
MP28 | Solyc10g086580.1.1 | Ribulose-1 5-bisphosphate carboxylase/oxygenase activase 1 | ||||
MP27 | Solyc11g006970.1.1 | Unknown protein DS12 from 2D-PAGE of leaf, chloroplastic | No data | No data | ||
MP30 | Solyc11g068510.1.1 | F1F0-ATPase inhibitor protein | AO up | No data | No data | |
MP35 | Solyc11g069000.1.1 | T-complex protein 1 subunit beta | −1.46 | 0.035 | ||
MP15 | Solyc12g056230.1.1 | Glutathione peroxidase | No data | No data |
The predicted Solyc (https://solgenomics.net/organism/Solanum_lycopersicum/genome; version 2.4 of the International Tomato Annotation Group, ITAG) is shown with the annotated function and significant difference between lines where applicable (5% significance level). The fold-change and p-value of the corresponding transcript, when present on the microarray and for p-values of < 0.05, is also shown for the AO-MDHAR lines and whether this fold-change is correlated with the difference seen in protein levels.