Table 4.
Loci significantly associated with Falling Numbers (FN), early preharvest sprouting (PHS) scores (days 3–4), 5 days of misting, late PHS scores (days 6–7), and PHS sprouting index (SI).
| QTLa | Marker | Chrb | cMb | –log10(p) | maf | Effectc | r2 | Environment | Favorable Alleled |
|---|---|---|---|---|---|---|---|---|---|
| QFN.wsu-4A* | IWB1884 | 4A | 152 | 6.63 | 0.48 | 10.28 | 0.00 | C16 FN | A/C |
| QFN.wsu-5A.1* | IWB60191 | 5A | 23 | 7.27 | 0.27 | 7.53 | 0.00 | P15 FN | A/G |
| QFN.wsu-5A.2 | IWB9800 | 5A | 141 | 7.77 | 0.20 | 7.43 | 0.00 | P15 FN | A/G |
| QFN.wsu-5D | IWB36060 | 5D | 202 | 6.11 | 0.35 | 11.70 | 0.08 | P13 FN | A/C |
| QFN.wsu-7A.1 | IWB22966 | 7A | 35 | 8.34 | 0.06 | 26.09 | 000 | P13 FN | A/G |
| QFN.wsu-7A.2 | IWA334 | 7A | 126 | 12.36 | 0.41 | 7.99 | 0.01 | P15 FN | A/C |
| QFN.wsu-7B.1 | IWB39063 | 7B | 162 | 7.91 | 0.48 | 10.88 | 0.01 | C16 FN | A/G |
| QFN.wsu-7B.2 | IWB75387 | 7B | – | 6.15 | 0.09 | 27.35 | 0.00 | P13 FN | A/C |
| QFN.wsu-unk | IWB37658 | unk | – | 6.82 | 0.09 | 15.49 | 0.00 | C16 FN | T/C |
| QPHS.wsu-1A.1 | IWB2320 | 1A | 82 | 6.73 | 0.15 | −0.04 | 0.00 | P14 d3 | T/C |
| QPHS.wsu-1A.2 | IWB6759 | 1A | 155 | 11.70 | 0.47 | −0.31 | 0.15 | P14 d3 | A/G |
| IWB77968 | 1A | 155 | 12.72 | 0.47 | −0.02 | 0.10 | P14 d3 | A/G | |
| QPHS.wsu-1B.2 | IWB64868 | 1B | 135 | 9.00 | 0.15 | −0.40 | 0.17 | C14 d4 | A/G |
| IWB31676 | 1B | 137 | 8.18 | 0.08 | −0.37 | 0.00 | P16 d3 | A/G | |
| QPHS.wsu-2A.1 | IWB42693 | 2A | 25 | 6.87 | 0.22 | −0.16 | 0.02 | C15 d3 | T/G |
| QPHS.wsu-2D | IWB7652 | 2D | 52 | 10.03 | 0.37 | −0.46 | 0.00 | C14 d4 | T/C |
| IWA8544 | 2D | 50 | 8.73 | 0.46 | −0.28 | 0.01 | P16 d3 | A/G | |
| IWA8544 | 2D | 50 | 9.12 | 0.46 | −0.32 | 0.07 | P16 d4 | A/G | |
| QPHS.wsu-3A.2 | IWB50719 | 3A | 68 | 6.71 | 0.14 | −0.29 | 0.04 | C14 d4 | A/G |
| QPHS.wsu-4A.1 | IWA7535 | 4A | 58 | 8.57 | 0.05 | −0.07 | 0.03 | P14 d3 | A/G |
| QPHS.wsu-4B.2 | IWB21707 | 4B | 75 | 8.93 | 0.10 | −0.42 | 0.07 | P14 d4 | A/G |
| QPHS.wsu-4B.3* | IWB22055 | 4B | 101 | 6.57 | 0.08 | −0.37 | 0.00 | P16 d3 | A/G |
| QPHS.wsu-5B.1 | IWB31067 | 5B | 26 | 8.75 | 0.08 | −0.06 | 0.01 | P14 d3 | T/G |
| QPHS.wsu-7B.1 | IWB54418 | 7B | 3 | 7.46 | 0.03 | −0.26 | 0.01 | P16 d3 | A/G |
| QPHS.wsu-1B.1* | IWB22868 | 1B | 31 | 7.88 | 0.18 | −0.30 | 0.01 | P14 d5 | T/C |
| QPHS.wsu-2D | IWB46396 | 2D | 54 | 9.63 | 0.39 | −0.49 | 0.02 | C14 d5 | A/G |
| QPHS.wsu-3B.2 | IWA6185 | 3B | 62 | 6.55 | 0.44 | −0.23 | 0.01 | P14 d5 | A/G |
| QPHS.wsu-4A.2 | IWB54609 | 4A | 66 | 7.30 | 0.17 | −0.35 | 0.01 | P16 d5 | A/G |
| QPHS.wsu-5A.2* | IWB10250 | 5A | 70 | 9.13 | 0.32 | −0.44 | 0.03 | P15 d5 | T/C |
| QPHS.wsu-5B.3 | IWB73511 | 5B | 129 | 6.73 | 0.30 | −0.28 | 0.01 | P16 d5 | A/G |
| QPHS.wsu-6B* | IWA1838 | 6B | 65 | 10.53 | 0.07 | −0.29 | 0.05 | P14 d5 | A/G |
| QPHS.wsu-7B.2* | IWB7099 | 7B | 133 | 9.00 | 0.00 | −0.34 | 0.00 | C14 d5 | A/G |
| QPHS.wsu-1D* | IWB71680 | 1D | 163 | 7.61 | 0.06 | −0.60 | 0.09 | P14, P16 d6 | A/G |
| QPHS.wsu-2A.2* | IWB17580 | 2A | 53 | 9.02 | 0.07 | −0.69 | 0.02 | C15 d7 | T/C |
| QPHS.wsu-2A.3 | IWB79387 | 2A | – | 6.88 | 0.01 | −0.31 | 0.00 | C14 d6 | A/G |
| QPHS.wsu-2D | IWB7652 | 2D | 52 | 12.69 | 0.37 | −0.85 | 0.12 | C14 d6, d7 | T/C |
| QPHS.wsu-3A.1 | IWB32631 | 3A | 15 | 6.63 | 0.26 | −0.31 | 0.02 | C14 d7 | A/G |
| QPHS.wsu-3B.1* | IWB6430 | 3B | 11 | 8.92 | 0.08 | −0.38 | 0.01 | P14 d7 | T/C |
| QPHS.wsu-3B.3 | IWB9902 | 3B | – | 7.76 | 0.07 | −0.59 | 0.06 | P14 d7 | T/C |
| QPHS.wsu-4A.2 | IWB46089 | 4A | 73 | 6.83 | 0.16 | −0.33 | 0.04 | P16 d6 | A/G |
| QPHS.wsu-4A.3 | IWB1389 | 4A | 151 | 7.51 | 0.23 | −0.45 | 0.02 | C14 d7 | T/G |
| QPHS.wsu-4B.1 | IWB72936 | 4B | 60 | 7.92 | 0.25 | −0.46 | 0.02 | C14 d7 | A/G |
| QPHS.wsu-4B.2 | IWA1382 | 4B | 73 | 8.04 | 0.06 | −0.53 | 0.00 | P15 d7 | A/G |
| QPHS.wsu-5A.2 | IWB60303 | 5A | 70 | 7.56 | 0.34 | −0.39 | 0.01 | C15 d7 | A/G |
| QPHS.wsu-5A.3* | IWB6049 | 5A | 84 | 9.92 | 0.19 | −0.31 | 0.00 | P16 d6 | A/G |
| QPHS.wsu-6A | IWB6726 | 6A | 77 | 7.52 | 0.07 | −0.48 | 0.03 | P14 d7 | T/G |
| QPHS.wsu-6B | IWB76583 | 6B | 65 | 9.76 | 0.05 | −0.33 | 0.03 | P14 d6 | A/G |
| QPHS.wsu-6D* | IWB49280 | 6D | 153 | 7.17 | 0.03 | −0.39 | 0.00 | P15 d7 | A/G |
| QPHS.wsu-7A* | IWB51129 | 7A | 152 | 6.64 | 0.00 | −0.27 | 0.06 | P16 d6 | A/G |
| QPHS.wsu-7B.3 | IWB10815 | 7B | 171 | 10.62 | 0.05 | −0.41 | 0.00 | P14 d7 | T/C |
| QPHS.wsu-1D* | IWB71680 | 1D | 163 | 7.22 | 0.06 | −0.03 | 0.10 | P16 SI | A/G |
| QPHS.wsu-2B | IWB30853 | 2B | 87 | 7.59 | 0.21 | −0.02 | 0.00 | C14 SI | A/G |
| QPHS.wsu-2D | IWB46396 | 2D | 54 | 11.97 | 0.39 | −0.03 | 0.07 | C14 SI | A/G |
| QPHS.wsu-3B.3 | IWB9902 | 3B | – | 7.31 | 0.07 | −0.03 | 0.00 | P14 SI | T/C |
| QPHS.wsu-5A.1 | IWB10998 | 5A | 53 | 8.70 | 0.41 | −0.02 | 0.25 | C14 SI | T/C |
| QPHS.wsu-6B | IWB57747 | 6B | 64 | 6.85 | 0.07 | −0.02 | 0.07 | P16 SI | A/G |
| IWB76583 | 6B | 65 | 6.57 | 0.02 | −0.01 | 0.00 | P14 SI | A/G | |
| QPHS.wsu-7B.2* | IWB7099 | 7B | 133 | 8.63 | 0.00 | −0.02 | 0.01 | C14 SI | A/G |
| IWB7099 | 7B | 133 | 7.58 | 0.01 | −0.02 | 0.00 | P16 SI | A/G |
a
QTL in bold explained 10% (r2 > 0.1) or more of the phenotypic variation. QTL underlined were significant in 2 environments. Loci more than 10 cM away from previously published QTL were considered to be novel and are indicated with an*.
b
Chromosome and position according to Wang et al. (2014). Positions are not reported if the location was identified on the GrainGenes database.
c
The allelic effect is shown in FN seconds or sprouting score BLUPs.
d
The significant allele is favorable (in bold) if it decreases sprouting scores in the spike-wetting tests or increases Falling Numbers.