Table 8.

Nonspecific cytotoxicity of selected compounds for murine fibroblast (L 929)

Formulate	L 929
	256 μΜ ± SD (N = 9)*	IC 50 (μΜ)†
CD 12	24.2 ± 0.9	3367.7‡
CD 24	29.5 ± 0.1	2931.9‡
CD 52	31.5 ± 0.4	2284.9‡
CD 45	44.0 ± 2.2	1581.4‡
CD 48	39.1 ± 6.3	2446.9‡
NLC 20’’	0 ± 0	1139.4
BNZ	3.9 ± 4.5	1436.9

BNZ = benznidazole; CD = cyclodextrin; L 929 = murine L-929 fibroblasts (NCTC clone 929); NLC = nanostructured lipid carriers; SD = standard deviation.

^*The results are expressed as the mean value of nonspecific cytotoxicity (growth inhibition) at the highest concentration tested (256 μΜ) ± SD (N = 9 each).

^†The concentration causing 50% growth inhibition was calculated using GraphPad Prism software.

^‡Significant differences (P < 0.05) compared with free BNZ (Student’s t test).