Table 5.
| A. Transcripts of Interest with Potentially Sex-Biased Expression in the A. leptorhynchus Pacemaker Nucleus | |||
|---|---|---|---|
| Short Annotation | Male RPKM | Female RPKM | IHW p-valuea |
| Hormone-Related Genes | |||
| 17β-HSD 12a | 6.0 ± 1.0 | 8.1 ± 0.8 | 0.024 |
| Estrogen Receptor α | 3.8 ± 2.5 | 1.0 ± 0.3 | 0.036 |
| Na+/K+ Channels | |||
| Kvβ2 (KCNAB2) | 170 ± 19 | 210 ±15 | 0.040 |
| K2p13.1 (KCNK13, THIK-1) | 37.3 ± 25.4 | 10.0 ±3.6 | 0.019 |
| BKβ4 (KCNMB4, Large conductance KCa β4) | 14.4 ± 5.9 | 4.1 ± 1.8 | 0.030 |
| KChIP4 (KCNIP4, K+ channel interacting protein 4) | 4.8 ± 2.3 | 11.5 ± 6.6 | 0.018 |
| Kir3.1 (KCNJ3, GIRK1) | 3.8 ± 1.1 | 8.1 ± 3.6 | 0.022 |
| Other Ion Channels/Transporters | |||
| TRPM2 (Transient receptor potential channel M2) | 10.0 ± 1.0 | 12.9 ± 0.8 | 0.014 |
| TRPM6 | 2.0 ± 0.0 | 3.6 ± 0.5 | 0.028 |
| Na+/K+ ATPase β3b | 3.9 ± 1.2 | 1.3 ± 0.3 | 0.033 |
| Neurotransmitters/Neuromodulators and Receptors | |||
| TARPγ2 (CACNG2) | 40.4 ± 3.8 | 49.9 ±2.5 | 0.019 |
| Neuropeptide Y Receptor Y8 | 10.1 ± 1.6 | 16.3 ±0.7 | 0.022 |
| GluR5 | 9.0 ± 0.6 | 13.1 ± 1.6 | 0.014 |
| Prolactin Releasing Peptide Receptor | 14.7 ± 1.4 | 5.8 ± 0.5 | <0.01 |
| GluR2 | 6.9 ± 0.4 | 9.2 ± 0.3 | 0.027 |
| Dopamine D2 Receptor | 10.4 ± 5.7 | 3.3 ± 1.5 | 0.04 |
| Nicotinic acetylcholine receptor α4 (nAChRα4) | 9.5 ± 4.2 | 2.2 ± 0.5 | <0.01 |
| Dopamine β-hydroxylase | 0.2 ± 0.1 | 1.3 ± 0.4 | 0.032 |
| B. Transcripts of Interest with Potentially Sex-Biased Expression in the A. albifrons Pacemaker Nucleus | |||
|---|---|---|---|
| Short Annotation | Male RPKM | Female RPKM | IHW p-valuea |
| Hormone-Related Genes | |||
| Prohibitin-1 | 108.5 ± 6.1 | 116.8 ± 9.2 | 0.029 |
| Nuclear Receptor Interacting Protein 1 (NRIP1) | 9.3 ± 0.9 | 6.9 ± 1.0 | 0.011 |
| Thyroid Hormone Receptor α | 16.1 ± 1.5 | 23.4 ± 2.4 | <0.001 |
| Na+/K+ Channels | |||
| Navβ1 (Danio rerio splice variant D, zβ1D, SCN1B) | 6.4 ± 0.9 | 3.6 ± 0.3 | 0.045 |
| K+ Channnel-Interacting Protein 2 (KChIP2, KCNIP2) | 10.4 ± 1.7 | 4.7 ± 1.0 | <0.001 |
| Kv6.3 (KCNG3) | 3.6 ± 0.5 | 6.6 ± 1.3 | <0.01 |
| Kv6.4 (KCNG4) | 2.3 ± .05 | 3.9 ± 0.5 | <0.01 |
| Kir3.1 (KCNJ3, GIRK1) | 3.0 ± 2.0 | 0.5 ± 0.2 | 0.022 |
| Kv1.2 (KCNA2) | 1.6 ± 0.3 | 0.5 ± 0.1 | <0.01 |
| Kv2.1 (KCNB1) | 1.5 ± 0.7 | 0.2 ± 0.2 | 0.032 |
| Kv1.1 (KCNA1) | 1.2 ± 0.7 | 0.1 ± 0.1 | 0.019 |
| Other Ion Channels/Transporters | |||
| Na+/K+ ATPase α3 | 22.3 ± 3.5 | 10.1 ± 0.7 | <0.001 |
| Acid-Sensing Ion Channel 1 (ASIC-1) | 9.9 ± 2.2 | 4.8 ± 0.5 | 0.011 |
| Cavβ2 (CACNB2, L-type Ca2+ channel β2 subunit) | 5.1 ±1.0 | 2.0 + 0.1 | <0.001 |
| Na+/K+ ATPase β2a | 4.9 ± 1.6 | 1.6 ± 0.3 | 0.030 |
| Na+/K+ ATPase α1a | 2.9 ± 0.7 | 0.9 ± 0.2 | 0.018 |
| Connexins | |||
| Connexin 32.2 (Astyanax mexicanus) | 140 ± 20 | 94 ± 8 | 0.011 |
| Gap Junction Protein β1 (Connexin 29/32) | 72.0 ± 4.6 | 83.5 ± 6.0 | <0.01 |
| Neurotransmitters/Neuromodulators and Receptors | |||
| Prepro-Neuropeptide Y | 27.2 ± 3.5 | 42.4 ± 7.9 | 0.011 |
| GABA Receptor β2 | 39.2 ± 3.2 | 27.2 ± 3.5 | 0.021 |
| Proenkephalin | 15.9 ± 5.1 | 4.9 ± 1.3 | 0.015 |
| Adenosine Receptor A1 | 23.6 ± 2.1 | 27.0 ± 3.6 | 0.047 |
| GABA Receptor ρ2 | 10.6 ± 3.4 | 3.6 ± 0.6 | <0.01 |
| Neuropeptide FF | 1.6 ± 1.2 | 11.0 ± 1.6 | <0.001 |
| Dopamine D2 Receptor | 9.1 ± 3.4 | 3.3 ± 1.3 | 0.031 |
| 5HT1B Receptor | 2.5 ± 0.5 | 4.8 ± 1.2 | 0.022 |
| 5HT1A Receptor | 3.4 ± 0.6 | 1.6 ± 0.4 | 0.017 |
| Substance P Receptor | 3.6 ±0.4 | 1.2 ± 0.3 | <0.001 |
| Dopamine D1B Receptor | 2.7 ± 1.1 | 0.4 ± 0.1 | 0.022 |
| VIP Receptor | 2.3 ± 1.3 | 0.2 ± 0.1 | 0.024 |
a
Independent Hypothesis-Weighting-corrected p-value with mean normalized read counts as a covariate (Ignatiadis et al., 2016).
a
Independent Hypothesis-Weighting-corrected p-value (Ignatiadis et al., 2016).