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. Author manuscript; available in PMC: 2018 Feb 19.
Published in final edited form as: Oncogene. 2015 Oct 12;35(23):3071–3078. doi: 10.1038/onc.2015.371

Figure 7.

Figure 7

ZBTB7A zinc finger mutant R399L stimulate glycolysis metabolism and promote cell proliferation. (a) Wild-type, R399L ZBTB7A and control vector were stably expressed in SW48 cells, the growth curve of these three cell lines are shown. Error bars indicate ± s.d. of three independent experiments. (b) Western blot analysis of the same cell lines as a, endogenous ZBTB7A and exogenous expressed Xpress-tagged ZBTB7A are separated and detected with anti-ZBTB7A antibody. β-Actin serves as loading control. (c) Glucose consumption and lactate production rates of cells lines stably express control vector, WT or R399L ZBTB7A. Average values of three independent experiments are shown, error bars indicate ± s.d. * indicates P < 0.05. (d) Growth curve of cell lines stably express control vector, WT or R399L ZBTB7A in the presence of glycolysis inhibitor 2-DG. Average values of three independent experiments are shown, error bars indicate ± s.d. (e) Nude mice tumor assay with SW48 cells stably express wild-type, R399L ZBTB7A or control vector, tumors of 24 days are shown. (f) Tumor volume was measured in subcutaneous tumors formed by wild-type, R399L ZBTB7A or control vector expressing SW48 cells. Data shown are mean ± s.d. of five tumors. 2-DG, 2-deoxyglucose.