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. 2018 Feb 1;145(3):dev159178. doi: 10.1242/dev.159178

Fig. 4.

Fig. 4.

Steroid-miR-125-Tom-Neur-Delta-Notch signaling cascade regulates reprogramming of the anterior TFC. (A) Confocal images and schematics of expression patterns of components from the steroid-miR-125-Tom-Neur-Delta-Notch signaling cascade in TFCs. Ecdysone-induced transcription factor BrZ1 (cyan) and let-7C miRNAs (including miR-125, yellow) are expressed in the posterior TFCs adjacent to the germline. The Notch antagonist Tom (magenta) has a reciprocal expression pattern; Tom is detected in the anterior TFCs, as assayed by the expression of Tom-GFP fusion protein, mRNA of which contains Tom-3′UTR with miR-125-binding site (see Materials and Methods). Ubiquitin ligase Neur (white) is expressed in the posterior TFC that also has Dl present in the vesicles. Neur expression was assayed by the expression of Neur-GFP fusion protein (see Materials and Methods). The anterior TFCs have higher Dl protein levels and no Notch activity, whereas the posterior TFCs adjacent to the germline have reduced Dl levels and are Notch active, except for the most posterior TFC that has vesicular Dl and no Notch activity. (B) Model proposing the role of the steroid-miR-125-Tom-Neur-Delta-Notch signaling cascade in the posterior TFC that allows its reprogramming into a Dl-sending state.