Fig. 2.

Absence of innervation of dorsal (peroneal) calf muscles in pma/pma homozygotes. Immunohistochemistry of cross-sections of stage-matched E16.5 wild-type (WT; left) and pma/pma homozygotes (right) using antibodies against myosin heavy chain to label muscle blocks (green) and β-III-tubulin to label nerves (red). (A,B) Low-magnification images with ventral muscles to the left and dorsal muscles to the right. (C,D) Higher-magnification images of dorsal muscle blocks showing innervation of wild-type dorsal muscles (C) but not of dorsal muscles in pma/pma homozygotes (D). (E,F) Magnification of ventral muscles showing innervation of both wild-type (E) and pma/pma (F) mice EDL, extensor digitorum longus; fib, fibula; PL, peroneus longus; TA, tibialis anterior; tib, tibia. Scale bars: 25 µm.