Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 1;11(1):dmm030544. doi: 10.1242/dmm.030544

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 1. — Transient ablation of CITED1+ NPCs triggers a compensatory increase in proliferation in surviving cells. (A) Schematic shows the stages at which tamoxifen was injected (i) and kidneys were harvested (h) for apoptosis analyses in WT (R26RDTA^het) and NPC^iDTA (Cited1-creER^T2;R26RDTA^het) mice. (B) Caspase3 (red, apoptosis) and cytokeratin 8 (CK8, green, collecting duct) immunostaining in kidneys injected at E12.5. White arrows point to Caspase3+, and F4/80+ cells in the CM (highlighted by dashed lines). Graph shows number of caspase3+ (E13.5) and TUNEL+ (E16.5) cells scored per cap mesenchyme (CM) per kidney section. (C) F4/80 (green, macrophages) and SIX2 (red, CM) staining. Number of F4/80+ cells per CM per kidney section is shown in the graph. (D) CITED1 (red, NPC marker) and CK8 (green, collecting duct) staining. Graph represents the number of CITED1+ NPCs estimated per kidney section. (E) Schematic shows the stages at which tamoxifen was injected (i) and kidneys were harvested (h) for proliferation analyses in WT and NPC^iDTA mice. (F) CITED1 (green), SIX2 (red) and pHH3 (cyan blue, proliferation marker) staining. Graphs show quantitation of CITED1+pHH3+ cells per kidney section. (G) Table shows the percentage of CITED1+ and SIX2+ cells remaining after 24 (E12.5→E13.5, E15.5→E16.5), 48 (E15.5→E17.5) and 72 (E12.5→E15.5) hours in NPC^iDTA kidneys relative to WT littermates. N indicates the number of mice analyzed at each time point. Data represent means±s.e.m. **P<0.005 and ***P<0.005, 2-tailed Student's t-test. Scale bars: 100 μm. cd, collecting duct; cm, cap mesenchyme. See also Fig. S1.