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. 2018 Jan 1;11(1):dmm030544. doi: 10.1242/dmm.030544

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Interstitial expansion is not associated with impaired proliferation-induced NPC depletion. (A) H&E-stained images of P10 NPC^Tak1 (Cited1-creER^T2;Tak1^c/c) and WT littermate controls (Tak1^c/c) tamoxifen treated at E15.5. (B,C) Kidney size and cortical thickness (in mm) of P10 WT and NPC^Tak1 kidneys. Number of individual tamoxifen-treated mice analyzed per group (n)=3. (D-G) PDGFRβ and αSMA (red; medullary interstitium) immunostaining in P10 WT and NPC^iDTA kidneys tamoxifen treated at E15.5. Graphs show quantitation of PDGFRβ+ and αSMA+ area (µm²) in the medullary region (indicated by brackets) per kidney section. (H,I) Co-staining of αSMA (red) with Ki67 (green; proliferation marker) in the medullary region. Number of αSMA+Ki67+ cells (white arrowheads) was scored per field per kidney section. Data represent means±s.e.m. N.S. not significant (P>0.05), *P<0.05, ***P<0.0005, 2-tailed Student's t-test. Scale bars: 500 μm (A), 200 μm (D,F) and 100 μm (G). See also Fig. S3.